The Eff ect of Sainfoin (Onobrychis viciifolia) Extract on Acethylcholine, Bethanechol and Potassium - Evoked Responses on Jejunum and Ileum of Sheep (1)

Summary The aim of this study was to clarify the eff ect of Onobrychis viciifolia Scop. (O. viciifolia) extract on jejunum and ileum of sheep The contractile response as E , pD and EC of acetylcholine (10 -3 -10 -8 -3 max 2 50 M, Ach), bethanechol (10 -10 -8 M) and potassium (10 - 80×10 -3 M, KCl) were determined in the absence and presence of extract (1.6 mg/ml). The contractile response to Ach in the presence of verapamil (10 -6 or 10 -8 M) or in calcium-free Tyrode’s solution was also determined in the absence and presence of extract. Cumulative treated of extract significantly reduced the response to KCl-evoked (10 -3 M) contraction. Extract did not aff ect contractile response to Ach and bethanechol but decreased the contractile response to potassium. The atropine-resistant component of Ach-evoked contraction and 4-diphenyl-acetoxy-N-methyl-piperidine methiodide-resistant component of bethanechol-evoked contraction were not inhibited in the presence of 1.6 mg/ml extract. The contractile response to Ach was reduced in calcium-free Tyrode’s solution and verapamil 10-8 M had no additional eff ect. In contrast to 1.6 mg/ml extract was added together with verapamil 10 -8 M, the contractile response to Ach was inhibited. In conclusion, extract inhibits jejunum and ileum muscle contractions through the inhibition of calcium infl ux and the modulation of calcium movement.