Cross‐reactivity of ciguatoxin, okadaic acid, and polyethers with monoclonal antibodies

The technology of the preparation of monoclonal antibodies to purified ciguatoxin (CTX) and okadaic acid (OA) are presented in this report. The cross‐reactivity of monoclonal antibody to CTX (MAb‐CTX) with purified OA (16%), ionomycin (2%) and ionophore A23187 (9%) are shown. Monoclonal antibody to OA (MAb‐OA) reacts with purified CTX (30%), while reaction with ionomycin (0.2%) and A23187 (3%) are minimal under the conditions examined. Thus, the cross‐reactivity of CTX and OA is demonstrated and in agreement with structural analysis. Furthermore, these MAbs are of importance in the detection of marine toxins in contaminated fishes.