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2012•Journal of VirologyOpen access

Reconstitution of the Entire Hepatitis C Virus Life Cycle in Nonhepatic Cells

Daniel Da Costa, Marine Turek, Daniel J. Felmlee, Erika Girardi, Sébastien Pfeffer, Gang Long, Ralf Bartenschlager, Mirjam B. Zeisel, Thomas F. Baumert

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Abstract

Hepatitis C virus (HCV) is a human hepatotropic virus, but the relevant host factors restricting HCV infection to hepatocytes are only partially understood. We demonstrate that exogenous expression of defined host factors reconstituted the entire HCV life cycle in human nonhepatic 293T cells. This study shows robust HCV entry, RNA replication, and production of infectious virus in human nonhepatic cells and highlights key host factors required for liver tropism of HCV.

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What this paper is about

Hepatitis C virus (HCV) is a human hepatotropic virus, but the relevant host factors restricting HCV infection to hepatocytes are only partially understood. We demonstrate that exogenous expression of defined host factors reconstituted the entire HCV life cycle in human nonhepatic 293T cells. This study shows robust HCV entry, RNA replication, and production of infectious virus in human nonhepatic cells and highlights key host factors required for liver tropism of HCV.

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OpenAlex reports 95 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

Hepatitis C virus (HCV) is a human hepatotropic virus, but the relevant host factors restricting HCV infection to hepatocytes are only partially understood. We demonstrate that exogenous expression of defined host factors reconstituted the entire HCV life cycle in human nonhepatic 293T cells. This study shows robust HCV entry, RNA replication, and production of infectious virus in human nonhepatic cells and highlights key host factors required for liver tropism of HCV.

Key concepts: Biology, Hepatitis C virus, Virology, Virus, Hepacivirus, Viral life cycle, Flaviviridae, Viral replication

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