1985•Journal of Clinical MicrobiologyOpen access

Isolation and enumeration of Clostridium botulinum by direct inoculation of infant fecal specimens on egg yolk agar and Clostridium botulinum isolation media

Constance Glasby, C L Hatheway

Abstract

Direct plating of stool specimens on selective (Clostridium botulinum isolation) and nonselective (egg yolk agar) media was evaluated as an aid in confirming infant botulism. C. botulinum was isolated from 13 of 14 culture-positive specimens with C. botulinum isolation and from 8 of 14 egg yolk agar. No lipase reaction was seen on plates of 31 culture-negative specimens.

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Key concepts: Clostridium botulinum, Isolation (microbiology), Microbiology, Agar, Botulism, Feces, Lecithinase, Yolk