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Preservation of the hyperthermophile Pyrococcus furiosus

Helen Connaris, Don A. Cowan, M. Ruffett, Richard Sharp

Open publisher page 5 citations

Abstract

Methods are evaluated for the preservation of the hyperthermophile Pyrococcus furiosus. The use of glass capillary tubes stored over liquid nitrogen with dimethyl sulphoxide appears to be the preferred method of preservation. Lyophilization resulted in loss of viability and storage at room temperature and +4°C resulted in considerable loss of viability within 4 weeks.

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What this paper is about

Methods are evaluated for the preservation of the hyperthermophile Pyrococcus furiosus. The use of glass capillary tubes stored over liquid nitrogen with dimethyl sulphoxide appears to be the preferred method of preservation. Lyophilization resulted in loss of viability and storage at room temperature and +4°C resulted in considerable loss of viability within 4 weeks.

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OpenAlex reports 5 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

Methods are evaluated for the preservation of the hyperthermophile Pyrococcus furiosus. The use of glass capillary tubes stored over liquid nitrogen with dimethyl sulphoxide appears to be the preferred method of preservation. Lyophilization resulted in loss of viability and storage at room temperature and +4°C resulted in considerable loss of viability within 4 weeks.

Key concepts: Pyrococcus furiosus, Hyperthermophile, Library science, Clinical microbiology, Archaea, Biology, Microbiology, Computer science

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