ScholarLens Back to search
1978•ScienceRequires access

Far-Ultraviolet Stopped-Flow Circular Dichroism

Jeremy Luchins, Sherman Beychok

Open publisher page 22 citations

Abstract

A stopped-flow circular dichroism instrument, with a total accessible wavelength range of 200 to 750 nanometers, has been constructed and provides a spectroscopic method for kinetic investigations of a wide array of fast reactions in which optical activity changes in absorbing regions are involved. An important biochemical application depends on the far-ultraviolet capability, which allows observation of the rapid alterations in backbone conformation associated with folding and unfolding reactions of proteins. Results obtained by following two such reactions at 222 nanometers represent direct monitoring by circular dichroism of rapid secondary structure changes in proteins.

About this research paper

What this paper is about

A stopped-flow circular dichroism instrument, with a total accessible wavelength range of 200 to 750 nanometers, has been constructed and provides a spectroscopic method for kinetic investigations of a wide array of fast reactions in which optical activity changes in absorbing regions are involved. An important biochemical application depends on the far-ultraviolet capability, which allows observation of the rapid alterations in backbone conformation associated with folding and unfolding reactions of proteins. Results obtained by following two such reactions at 222 nanometers represent direct monitoring by circular dichroism of rapid secondary structure changes in proteins.

Why it matters

OpenAlex reports 22 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

A stopped-flow circular dichroism instrument, with a total accessible wavelength range of 200 to 750 nanometers, has been constructed and provides a spectroscopic method for kinetic investigations of a wide array of fast reactions in which optical activity changes in absorbing regions are involved. An important biochemical application depends on the far-ultraviolet capability, which allows observation of the rapid alterations in backbone conformation associated with folding and unfolding reactions of proteins. Results obtained by following two such reactions at 222 nanometers represent direct monitoring by circular dichroism of rapid secondary structure changes in proteins.

Key concepts: Circular dichroism, Ultraviolet, Far ultraviolet, Folding (DSP implementation), Nanometre, Chemistry, Stopped flow, Protein folding

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Far-Ultraviolet Stopped-Flow Circular Dichroism — Research Paper | ScholarLens