Reactivity Of Brucella abortus Strain 19 Outer Membrane Protein Toward Polyclonal Antibody Anti Brucella abortus Strain 19

The aim of this research was to find out the reactivity of Brucella abortus S-19 outer membrane protein toward rabbit polyclonal antibody which have been injected with Brucella abortus S-19. In advanced it will be used as diagnostic kit that has sensitivity and highly specific for Brucellosis diagnosis.Brucella abortus S-19 was obtained from PUSVETMA bacteria stock. The suspention consist of Brucella abortus S-19 and the adjuvant was injected subcutaneous into rabbit in order to get polyclonal antibody anti Brucella abortus S-19. Booster has been done for six times.OMP was obtained from Brucella abortus S-19 by sonication technique, using ultrasonic homogenizer. The identification of immunogenic antigen protein fraction had done by western blot. Western blot technique used OMP antigen and polyclonal antibody (anti Brucella abortus S-19).The result of this research showed that OMP Brucella abortus S-19 have had five fractions. These protein had recognized antibody with molecul weight of 55,3 kDa, 24,4 kDa, 21,9 kDa, 20,3 kDa and 14,7 kDa.