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2011•African Journal of Pharmacy and PharmacologyOpen access

The traditional Chinese medicine Huang-Lian-Jie-Du-Tang inhibits hypoxia- induced neuronal apoptosis

Qichun Zhang

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Abstract

Huang-Lian-Jie-Du-Tang (HLJDT) has been used clinically for cerebral ischemia therapeutics. Here, we aimed to demonstrate whether and how HLJDT regulates neuronal apoptosis under hypoxia/ischemia. Apoptosis analysis was performed in vitro in PC12 cells with flow cytometry and in vivo in MCAO rats using TUNEL staining. The levels of caspase 9, caspase 3, Bcl-2, Bax and HIF-1α were demonstrated with western blotting. HLJDT remarkably inhibited apoptosis of neurons, both in vitro and in vivo. Caspase 9, caspase 3m Bcl-2, Bax and HIF-1α play important roles in anti-apoptotic effect of HLJDT on neuronal apoptosis hypoxia and ischemia induced.

About this research paper

What this paper is about

Huang-Lian-Jie-Du-Tang (HLJDT) has been used clinically for cerebral ischemia therapeutics. Here, we aimed to demonstrate whether and how HLJDT regulates neuronal apoptosis under hypoxia/ischemia. Apoptosis analysis was performed in vitro in PC12 cells with flow cytometry and in vivo in MCAO rats using TUNEL staining. The levels of caspase 9, caspase 3, Bcl-2, Bax and HIF-1α were demonstrated with western blotting. HLJDT remarkably inhibited apoptosis of neurons, both in vitro and in vivo. Caspase 9, caspase 3m Bcl-2, Bax and HIF-1α play important roles in anti-apoptotic effect of HLJDT on neuronal apoptosis hypoxia and ischemia induced.

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OpenAlex reports 8 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

Huang-Lian-Jie-Du-Tang (HLJDT) has been used clinically for cerebral ischemia therapeutics. Here, we aimed to demonstrate whether and how HLJDT regulates neuronal apoptosis under hypoxia/ischemia. Apoptosis analysis was performed in vitro in PC12 cells with flow cytometry and in vivo in MCAO rats using TUNEL staining. The levels of caspase 9, caspase 3, Bcl-2, Bax and HIF-1α were demonstrated with western blotting. HLJDT remarkably inhibited apoptosis of neurons, both in vitro and in vivo. Caspase 9, caspase 3m Bcl-2, Bax and HIF-1α play important roles in anti-apoptotic effect of HLJDT on neuronal apoptosis hypoxia and ischemia induced.

Key concepts: Traditional Chinese medicine, Traditional medicine, Hypoxia (environmental), Apoptosis, Medicine, Pharmacology, Chemistry, Biochemistry

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