2005•Applied and Environmental MicrobiologyOpen access

Improved System for Construction and Analysis of Single-Copy β-Galactosidase Operon Fusions in Yersinia enterocolitica

Michelle E. Maxson, Andrew J. Darwin

Abstract

We report a significantly improved system for studying single-copy lacZ operon fusions in Yersinia enterocolitica: a simple procedure for the stable integration of lacZ operon fusions into the ara locus and a strain with a deletion mutation that abolishes the low level of endogenous beta-galactosidase activity.

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Key concepts: Yersinia enterocolitica, Operon, L-arabinose operon, lac operon, gal operon, Biology, Locus (genetics), Enterobacteriaceae