1996•Journal of Clinical MicrobiologyOpen access

Rapid detection and isolation of shiga-like toxin (verocytotoxin)-producing Escherichia coli by direct testing of individual enterohemolytic colonies from washed sheep blood agar plates in the VTEC-RPLA assay

Lothar Beutin, Sabine Zimmermann, Kerstin Gleier

Abstract

By combining the enterohemolysin test and the VTEC-RPLA test (specific for the detection of Shiga-like toxin I [SLT-I], SLT-II, and SLT-IIc), single colonies of SLT-producing Escherichia coli were found to constitute between 0.03 and 68.1% of the coliform flora in human stool cultures and were isolated and characterized within 72 to 96 h.

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Key concepts: Verocytotoxin, VTEC, Shiga-like toxin, Microbiology, Escherichia coli, Shiga toxin, Agar, Toxin