1984Journal of BacteriologyOpen access

Effects of deletions in transposon Tn7 on its frequency of transposition

G M Smith, Peter Jones

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Abstract

Deletions in transposon Tn7 either abolished transposition or reduced transposition frequency. Except for a deletion in the right-hand terminus, these deletions could be complemented in trans. A 2.1-kilobase fragment of Tn7 encodes a diffusible gene product which stimulates transposition above the wild-type frequency. No cointegrate formation was detected.

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Deletions in transposon Tn7 either abolished transposition or reduced transposition frequency. Except for a deletion in the right-hand terminus, these deletions could be complemented in trans. A 2.1-kilobase fragment of Tn7 encodes a diffusible gene product which stimulates transposition above the wild-type frequency. No cointegrate formation was detected.

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Available abstract

Deletions in transposon Tn7 either abolished transposition or reduced transposition frequency. Except for a deletion in the right-hand terminus, these deletions could be complemented in trans. A 2.1-kilobase fragment of Tn7 encodes a diffusible gene product which stimulates transposition above the wild-type frequency. No cointegrate formation was detected.

Key concepts: Transposition (logic), Transposable element, Biology, Genetics, Transposase, Gene, Molecular biology, Mutant

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