Isolation and characterization of microsatellite markers from Fangzheng silver crucian carp,Carassius auratus gibelio(Bloch), and cross‐amplification in the closely related species crucian carp,Carassius auratus auratus(Linnaeus)

Abstract We report the development of 20 microsatellite markers for Fangzheng silver crucian carp,Carassius auratus gibelio(Bloch). Nineteen out of 20 showed polymorphism with alleles ranging from two to 14. These loci were screened to amplify the closely related species crucian carp,Carassius auratus auratus(Linnaeus) and all of them can amplify DNA with the size similar to the former. The origin of silver crucian carp is in issue and the population genetic structure is still unclear. Microsatellite markers isolated from the silver crucian carp and their utility in the crucian carp will be useful for these researches.