2007•Italian Journal of Animal ScienceOpen access

Genetic characterization of indigenous anatolian water buffalo breed using microsatellite dna markers

Mehmet İhsan SOYSAL, E. Özkan, Süleyman Kök, M. Occidente, Y. T. Tuna, Eser Kemal Gürcan, Donato MATASSINO

Abstract

One indigenous water buffalo population to Anatolia was characterised with 11 cattle autosomal microsatellite loci. A set of 4 cattle microsatellite loci was found to be polymorphic in the Anatolian buffalo genome. Genotyping of these polymorphic microsatellite loci revealed alleles ranging from 3 to 9. The observed heterozygosity ranged from 0.550 to 0.775 and the expected heterozygosity ranged from 0.494 to 0.815. The Fis value within each locus, changed from –0.101 to 0.205. Total Fis was 0.043 indicating that Anatolian water buffalo population samples seemed to be in Hardy- Weinberg expectation.

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Key concepts: Microsatellite, Loss of heterozygosity, Biology, Genotyping, Locus (genetics), Breed, Genetics, Population