Biotransformation of Oleic Acid byMicrococcus luteusCells
Nobuyoshi Esaki, Shigeki Ito, Wolfgang A Blank, Kenji Soda
Abstract
Nobuyoshi Esaki, Shigeki Ito, Wolfgang A Blank, Kenji Soda
Abstract
We isolated bacterial strains tolerant to high concentrations of oleic acid from soil samples, and studied biotransformation of fatty acids with the bacteria. We found that a strain BL0–3 identified as Micrococcus luteus metabolized oleic acid to 10-hydroxystearic acid, 10-oxostearic acid, and 4-oxolauric acid. Palmitoleic acid and myristoleic acid also served as substrates in the biotransformation, and 10-oxopalmitic acid and 10-oxomyristic acid were produced, respectively. The formation of 4-oxolauric acid from oleic acid was inhibited by compounds which inhibit β-oxidation of fatty acids.
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We isolated bacterial strains tolerant to high concentrations of oleic acid from soil samples, and studied biotransformation of fatty acids with the bacteria. We found that a strain BL0–3 identified as Micrococcus luteus metabolized oleic acid to 10-hydroxystearic acid, 10-oxostearic acid, and 4-oxolauric acid. Palmitoleic acid and myristoleic acid also served as substrates in the biotransformation, and 10-oxopalmitic acid and 10-oxomyristic acid were produced, respectively. The formation of 4-oxolauric acid from oleic acid was inhibited by compounds which inhibit β-oxidation of fatty acids.
Key concepts: Biotransformation, Oleic acid, Palmitoleic acid, Micrococcus luteus, Chemistry, Fatty acid, Bacteria, Biochemistry