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1996•Journal of Clinical MicrobiologyOpen access

Sensitive PCR diagnosis of Infections by Enterocytozoon bieneusi (microsporidia) using primers based on the region coding for small-subunit rRNA

Alexandre J. da Silva, David A. Schwartz, Govinda S. Visvesvara, Hércules Moura, Susan B. Slemenda, Norman J. Pieniążek

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Abstract

Enterocytozoon bieneusi is the most common microsporidian infecting patients with AIDS. We have developed a PCR primer pair, named EBIEF1/EBIER1, based on the small-subunit rRNA sequence of this microsporidian. Compared with other PCR-based methods, this primer pair shows a higher efficiency of detection in diagnostic applications than does another previously described primer pair, V1/EB450.

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What this paper is about

Enterocytozoon bieneusi is the most common microsporidian infecting patients with AIDS. We have developed a PCR primer pair, named EBIEF1/EBIER1, based on the small-subunit rRNA sequence of this microsporidian. Compared with other PCR-based methods, this primer pair shows a higher efficiency of detection in diagnostic applications than does another previously described primer pair, V1/EB450.

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OpenAlex reports 127 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

Enterocytozoon bieneusi is the most common microsporidian infecting patients with AIDS. We have developed a PCR primer pair, named EBIEF1/EBIER1, based on the small-subunit rRNA sequence of this microsporidian. Compared with other PCR-based methods, this primer pair shows a higher efficiency of detection in diagnostic applications than does another previously described primer pair, V1/EB450.

Key concepts: Enterocytozoon bieneusi, Microsporidia, Microsporidiosis, Biology, Primer (cosmetics), Ribosomal RNA, Polymerase chain reaction, Virology

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Sensitive PCR diagnosis of Infections by Enterocytozoon bieneusi (microsporidia) using primers based on the region coding for small-subunit rRNA — Research Paper | ScholarLens