DEVELOPMENT AND VALIDATION OF NOVEL HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF CEFIXIME AND MOXIFLOXACIN IN COMBINED TABLET DOSAGE FORM

A simple, rapid, accurate and precise RP-HPLC method has been developed and validated for simultaneous estimation of Cefixime and Moxifloxacin in combined tablet dosage form. The chromatographic separation was carried out on Hypersil BDS C18 column (100 x 4.6 mm; 3 µ) with a mixture of phosphate buffer pH 6.0: acetonitrile (75: 25 V/V) as a mobile phase; at a flow rate of 1.0 mL/min. UV detection was performed at 293 nm. The retention times were 2.374 min and 5.776 min for Cefixime and Moxifloxacin respectively. Calibration plots were linear (r 2 =0.999) over the concentration range of 5-30 µg/mL for both Cefixime and Moxifloxacin. The method was validated for linearity, accuracy, precision, specificity and sensitivity. The proposed method was successfully used for quantitative analysis of Cefixime and Moxifloxacin tablets. No interference from any component of pharmaceutical dosage form was observed. Validation studies revealed that the method is specific, rapid, reliable and reproducible. The high recovery and low relative standard deviation confirm the suitability of the proposed method for routine estimation of Cefixime and Moxifloxacin in pure sample and tablet dosage forms.