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Construction of a Restriction Map of the Gluconobacter Bacteriophage A-1 Genome

Nikolaos K. Robakis, Norberto J. Palleroni, M. Boublík, Carl W. Despreaux

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Abstract

Summary: The molecular weight of Gluconobacter bacteriophage A-1 DNA was determined by restriction analysis and electron microscopic measurements to be about 30 × 106. The DNA has cohesive ends and forms ring-like monomers. A circular map with the relative positions of the cleavage sites for the restriction enzymes EcoRI, SmaI, XhoI and BamHI is proposed.

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Summary: The molecular weight of Gluconobacter bacteriophage A-1 DNA was determined by restriction analysis and electron microscopic measurements to be about 30 × 106. The DNA has cohesive ends and forms ring-like monomers. A circular map with the relative positions of the cleavage sites for the restriction enzymes EcoRI, SmaI, XhoI and BamHI is proposed.

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Available abstract

Summary: The molecular weight of Gluconobacter bacteriophage A-1 DNA was determined by restriction analysis and electron microscopic measurements to be about 30 × 106. The DNA has cohesive ends and forms ring-like monomers. A circular map with the relative positions of the cleavage sites for the restriction enzymes EcoRI, SmaI, XhoI and BamHI is proposed.

Key concepts: XhoI, EcoRI, BamHI, Restriction enzyme, Restriction map, DNA, Biology, Bacteriophage

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