2006•Antimicrobial Agents and ChemotherapyOpen access
High Prevalence of the K65R Mutation in Human Immunodeficiency Virus Type 1 Subtype C Isolates from Infected Patients in Botswana Treated with Didanosine-Based Regimens
Florence Doualla‐Bell, Ava Avalos, Bluma Brenner, Tendani Gaolathe, Madisa Mine, Simani Gaseitsiwe, Maureen Oliveira, Daniella Moisi, Ndwapi Ndwapi, Howard Moffat, Max Essex, Mark A. Wainberg
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Abstract
We analyzed the reverse transcriptase genotypes of human immunodeficiency virus type 1 subtype C viruses isolated from 23 patients in Botswana treated with didanosine-based regimens. The K65R mutation was selected either alone or together with the Q151M, S68G, or F116Y substitution in viruses from seven such individuals. The results of in vitro passage experiments were consistent with an apparent increased propensity of subtype C viruses to develop the K65R substitution.