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BucolomeN-glucuronide: Purification and identification of a major metabolite of bucolome in rat bile

Kiminori Mohri, Takashi Uesugi, Kazuaki KAMISAKA

Abstract

A biliary metabolite of bucolome in rat was isolated and purified by column chromatography. The metabolite has been identified as bucolome N3-glucuronide by chemical and spectroscopic data. This study is the first to show an N-glucuronide to be the major metabolite of barbiturates in rat bile.

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Key concepts: Metabolite, Glucuronide, Chemistry, Chromatography, Column chromatography, Biochemistry