1996•Journal of Clinical MicrobiologyOpen access

Isolation of group B porcine rotavirus in cell culture

Takeshi Sanekata, Yuhei Kuwamoto, Sakura Akamatsu, Naomi Sakon, M. Oseto, K. Taniguchi, Shuji Nakata, M K Estes

Abstract

While group A and C rotaviruses have been grown in cell culture, group B rotavirus has never been cultured. In this study we successfully isolated porcine group B rotavirus in swine kidney cells. Pancreatin treatment is essential for the propagation of group B rotavirus.

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Key concepts: Rotavirus, Group A, Group B, Reoviridae, Virology, Cell culture, Biology, Isolation (microbiology)