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2009•Journal of BiopesticidesOpen access

BIOEFFICACY OF CRUDE PROTEIN OF NATIVE BACILLUS THURINGIENSIS (BERLINER) ISOLATES AGAINST CABBAGE LEAF WEBBER, CROCIDOLOMIA BINOTOLIS ZEL.

C. J. Nethravathi

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Abstract

ABSTRACT Investigations were carried out to assess the efficacy of crude protein of some of Bacillus thuringiensis isolates against lepidopteran pest viz., cabbage leaf webber (CLW) Crocidolmia binotalis Zel. mortality. The native isolate 1526B/b recorded highly virulent (LC50 = 682.420 ppm) where as M-126 recorded lowest virulent (LC50 = 6572.822 ppm). To identify the spectrum of insecticidal genes in the native B. thuringiensis isolates, the polymerase chain reaction (PCR) was carried out and the results show that none of the isolates amplified cry3, 4 and 18 genes.

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ABSTRACT Investigations were carried out to assess the efficacy of crude protein of some of Bacillus thuringiensis isolates against lepidopteran pest viz., cabbage leaf webber (CLW) Crocidolmia binotalis Zel. mortality. The native isolate 1526B/b recorded highly virulent (LC50 = 682.420 ppm) where as M-126 recorded lowest virulent (LC50 = 6572.822 ppm). To identify the spectrum of insecticidal genes in the native B. thuringiensis isolates, the polymerase chain reaction (PCR) was carried out and the results show that none of the isolates amplified cry3, 4 and 18 genes.

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Available abstract

ABSTRACT Investigations were carried out to assess the efficacy of crude protein of some of Bacillus thuringiensis isolates against lepidopteran pest viz., cabbage leaf webber (CLW) Crocidolmia binotalis Zel. mortality. The native isolate 1526B/b recorded highly virulent (LC50 = 682.420 ppm) where as M-126 recorded lowest virulent (LC50 = 6572.822 ppm). To identify the spectrum of insecticidal genes in the native B. thuringiensis isolates, the polymerase chain reaction (PCR) was carried out and the results show that none of the isolates amplified cry3, 4 and 18 genes.

Key concepts: Bacillus thuringiensis, Biology, Virulence, PEST analysis, Polymerase chain reaction, Veterinary medicine, Gene, Botany

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BIOEFFICACY OF CRUDE PROTEIN OF NATIVE BACILLUS THURINGIENSIS (BERLINER) ISOLATES AGAINST CABBAGE LEAF WEBBER, CROCIDOLOMIA BINOTOLIS ZEL. — Research Paper | ScholarLens