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2007•Chinese Journal of Agricultural BiotechnologyRequires access

Real-time quantitative PCR detection ofEscherichia coliO157:H7

Si Chen, Huang Kun-Lun, Wentao Xu, Yuan Li, Luo Yun-bo

Open publisher page 10 citations

Abstract

Abstract A rapid and accurate real-time quantitative polymerase chain reaction (real-time PCR) method with SYBR Green I was established for detectingEscherichia coliO157:H7. A pair of primers were designed to amplify theeaegene. The dissociation curves showed that the amplification product was very specific. The optimal conditions and standard curve were established. The result indicated that real-time PCR was 1000 times more sensitive than ordinary PCR.

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What this paper is about

Abstract A rapid and accurate real-time quantitative polymerase chain reaction (real-time PCR) method with SYBR Green I was established for detectingEscherichia coliO157:H7. A pair of primers were designed to amplify theeaegene. The dissociation curves showed that the amplification product was very specific. The optimal conditions and standard curve were established. The result indicated that real-time PCR was 1000 times more sensitive than ordinary PCR.

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OpenAlex reports 10 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

Abstract A rapid and accurate real-time quantitative polymerase chain reaction (real-time PCR) method with SYBR Green I was established for detectingEscherichia coliO157:H7. A pair of primers were designed to amplify theeaegene. The dissociation curves showed that the amplification product was very specific. The optimal conditions and standard curve were established. The result indicated that real-time PCR was 1000 times more sensitive than ordinary PCR.

Key concepts: Real-time polymerase chain reaction, Escherichia coli, Polymerase chain reaction, SYBR Green I, Biology, Standard curve, Melting curve analysis, Molecular biology

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