Transposon Tn10 provides a promoter for transcription of adjacent sequences.
M. Sofia Ciampi, Molly B. Schmid, J R Roth
Abstract
M. Sofia Ciampi, Molly B. Schmid, J R Roth
Abstract
Promoters located within the Tn10 insertion element cause transcription of "host" sequences adjacent to both ends of the inserted Tn10 element. These promoters are usually not observed in genetic experiments because their transcripts are efficiently terminated at nearby rho-dependent termination sites. The observations presented here provide an explanation for several confusing aspects of transposon behavior and suggest the possibility that many transposons possess promoters that have escaped detection for similar reasons.
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Promoters located within the Tn10 insertion element cause transcription of "host" sequences adjacent to both ends of the inserted Tn10 element. These promoters are usually not observed in genetic experiments because their transcripts are efficiently terminated at nearby rho-dependent termination sites. The observations presented here provide an explanation for several confusing aspects of transposon behavior and suggest the possibility that many transposons possess promoters that have escaped detection for similar reasons.
Key concepts: Transposable element, Tn10, Promoter, Genetics, DNA Transposable Elements, Biology, Transcription (linguistics), Gene