ScholarLens Back to search
1990•Journal of the Chemical Society Chemical CommunicationsRequires access

A sensitive method for the determination of the primary amide function (RCONH2) in peptides by mass spectrometry

Jennifer C. Nutkins, Dudley H. Williams

Open publisher page 3 citations

Abstract

Several model peptides have been trimethylsilylated and their FIB mass spectra obtained; reaction was detected at hydroxy groups and primary amides only, amines and carboxy groups were observed in underivatised form, and thus, in conjunction with standard procedures for the determination of hydroxy groups, this methodology represents a sensitive and extremely rapid means for the detection of primary amides in such molecules.

About this research paper

What this paper is about

Several model peptides have been trimethylsilylated and their FIB mass spectra obtained; reaction was detected at hydroxy groups and primary amides only, amines and carboxy groups were observed in underivatised form, and thus, in conjunction with standard procedures for the determination of hydroxy groups, this methodology represents a sensitive and extremely rapid means for the detection of primary amides in such molecules.

Why it matters

OpenAlex reports 3 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Several model peptides have been trimethylsilylated and their FIB mass spectra obtained; reaction was detected at hydroxy groups and primary amides only, amines and carboxy groups were observed in underivatised form, and thus, in conjunction with standard procedures for the determination of hydroxy groups, this methodology represents a sensitive and extremely rapid means for the detection of primary amides in such molecules.

Key concepts: Primary (astronomy), Chemistry, Amide, Mass spectrometry, Protein primary structure, Function (biology), Peptide, Chromatography

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
A sensitive method for the determination of the primary amide function (RCONH2) in peptides by mass spectrometry — Research Paper | ScholarLens