1992•The Plant JournalRequires access
Random sequencing of cDNA libraries reveals a variety of expressed genes in cultured cells of rice (Oryza sativa L.)
Hirofumi Uchimiya, Shin‐ichiro Kidou, Tetsuo Shimazaki, Satoshi Aotsuka, Susumu Takamatsu, Ritsuko Nishi, Hiroshi Hashimoto, Yuko Matsubayashi, Naomi Kidou, Masaaki Umeda, Atsushi Kato
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Abstract
Summary Large scale sequencing of randomly selected cDNA clones was carried out to investigate the feasibility of this method for isolating plant genes. cDNA libraries were made using mRNA prepared from suspension‐cultured cells of rice (Oryza sativa L.). Partial nucleotide sequences of 830 individual cDNA clones have been determined and compared with the GenBank database. Approximately 8% of the cDNA clones could be identified as particular genes. This method provides the opportunity to isolate large numbers of plant genes.