1984•Scandinavian Journal of ImmunologyRequires access
The Appearance of Cytotoxic Lymphocytes in Unstimulated and Concanavalin A‐Activated Human Peripheral Mononuclear Cells
U Persson, S. G. O. Johansson
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Abstract
The presence of a mitogenic lectin during the cytotoxicity assay enables the detection of non-specific cytotoxicity (lectin-dependent cell-mediated cytotoxicity (LDCC]. Human mononuclear cells cultured at 37 degrees C in vitro became cytotoxic after 1 day, as detected by the LDCC. In some individuals, the presence of adherent cells during culture inhibited the development of cytotoxicity. Activation by concanavalin A (Con A) resulted in enhanced cytotoxicity after a few days in culture. There was no clear relationship between the lectin doses required for optimal induction of cytotoxicity and [3H]thymidine uptake. High Con A concentrations that induced a proliferative response often inhibited cytotoxicity in mononuclear cells depleted of adherent cells.