2007•Journal of Clinical MicrobiologyOpen access
Increased Detection of Respiratory Syncytial Virus, Influenza Viruses, Parainfluenza Viruses, and Adenoviruses with Real-Time PCR in Samples from Patients with Respiratory Symptoms
Alma C. van de Pol, Anton M. van Loon, Tom F.W. Wolfs, Nicolaas J. G. Jansen, Monique Nijhuis, Els Klein Breteler, Rob Schuurman, John W. A. Rossen
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Abstract
Respiratory samples (n = 267) from hospitalized patients with respiratory symptoms were tested by real-time PCR, viral culture, and direct immunofluorescence for respiratory syncytial virus, influenza virus, parainfluenza viruses, and adenoviruses. Compared with conventional diagnostic tests, real-time PCR increased the diagnostic yields for these viruses from 24% to 43% and from 3.5% to 36% for children and adults, respectively.