1999•Clinical and Diagnostic Laboratory ImmunologyOpen access

A Novel Enzyme-Linked Immunosorbent Assay Using the RecombinantActinobacillus pleuropneumoniaeApxII Antigen for Diagnosis of Pleuropneumonia in Pig Herds

Gero Leiner, B Franz, K Strutzberg, Gerald-F. Gerlach

Abstract

For the surveillance of pig herds infected with porcine pleuropneumonia, an enzyme-linked immunosorbent assay (ELISA) using the recombinant Actinobacillus pleuropneumoniae ApxII protein as species- but not serotype-specific antigen was developed. Using this ELISA, 243 of 400 animals from 22 A. pleuropneumoniae-infected herds were classified as seropositive.

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Available abstract

Key concepts: Actinobacillus pleuropneumoniae, Pleuropneumonia, Serotype, Antigen, Recombinant DNA, Microbiology, Herd, Virology