Evaluation of the Heatsynch protocol in Murrah buffaloes (Bubalus bubalis) during hot summer season

The objective of this study was to evaluate the effect of the Heatynch protocol on plasma estrogen concentration in Murrah buffaloes and to evaluate if the Heatsynch protocol induces estrus in Murrah buffaloes. All treated animals responded to the treatment by displaying obvious signs of estrus. The mean plasma estrogen concentration among the individual buffalo was 191.9 ± 68.3 pg/ml after Estradiol benzoate (EB) injection. The plasma progesterone profile of these animals indicated that out of 16 animals 8 animals were acyclic before Heatsynch treatment (8/16) as plasma progesterone concentrations in these animals were basal (<0.24±0.01 ng/ml) for most of the sampling period during the course of treatment. Following Heatsynch treatment only two buffalo out of sixteen were acyclic (2/16) on the basis of their plasma progesterone profile. These results indicated that Heatsynch protocol is useful in inducing cyclicity in acyclic buffaloes. The seven buffalo out of sixteen (7/16) were conceived after treatment with Heatsynch protocol in summer. So, the treatment might have potential field application value to overcome poor estrus symptoms in summer.