An optical measurement using the DNA hybridization method for specific species of bacteria detection

This paper reports a developed DNA hybridization detection method using gold-streptavidin conjugates as a reporter, the silver enhancement reaction to magnify the detection signal, and a flatbed scanner to detect the hybridization signal. There was a significant difference in grayscale between the experimental samples and the negative control after 18 min. The detection limit for a DNA sample is 8.25 ng/mL with 5 µM of probe and 10−3µM of AuNPsstreptavidin. The probe specific tests for different DNA samples were discussed: (1) the probe specific tests for various species of bacteria, and (2) the probe specific tests between the various strains of a single species and various species of a single genus. The developed DNA hybridization method is easy, convenient, and rapid.