Repeatability and Reproducibility of A-Scan Biometry Quantitative Findings

PURPOSE: To determine repeatability and reproducibility of A-scan biometry quantitative findings in order to uniformly evaluate lenticular cataract changes. MATERIALS AND METHODS: Seventeen eyes from seventeen cataract patients were examined by three (trainee ophthalmologists). Results (ratio of mean internal lens spikes height/lens anterior surface spike height) were compared using coefficient of variation (CV), repeatability coefficient (CR), and intraclass correlation coefficients (ICC). RESULTS: Intra-observer consistency was CV = 0.36, CR = 0.49, and ICC = 0.778 for the first operator; CV = 0.33, CR = 0.52, and ICC = 0.642 for the second operator; and CV = 0.32, CR = 0.53, and ICC = 0.567 for the third operator. Concerning inter-observer agreement: CV (in 6 of 7 eyes) was > 8%, CR was > 0.21, and intraclass correlation coefficient for all eyes was < 0.81. CONCLUSIONS: Regarding repeatability and reproducibility, although there is a tendency for statistical significance, variability of the results does not allow the use of the method as a complementary clinical tool for comparing results. Standardization of this procedure, in order to achieve higher test reliability, might be the aim of relevant future studies, although there are a number of points that have to be addressed.