2008•Unpublished venueRequires access
SURVEY OF TOMATO YELLOW LEAF CURL DISEASE-ASSOCIATED VIRUSES IN THE EASTERN MEDITERRANEAN BASIN
Ghandi H. Anfoka, Mohammad K. Abhary, Fatima Haj Ahmad, Anaam Fuad Hussein, Adel Abdel-Sabour REZK, Fouad Akad, Yusuf Abou-Jawdah, Moshe Lapidot, F. Vidavski, Mark K. Nakhla, Hana Sobh, Hagop S. Atamian, Lidya Cohen, Iris Sobol, H. M. Mazyad, Douglas P. Maxwell, Henryk Hanokh Czosnek
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Abstract
Tomato production in the Mediterranean region and elsewhere is under constant threat of the whitefly-transmitted begomoviruses that cause Tomato yellow leaf curl disease (TYLCD). Sequencing has indicated that the generic ‘TYLCV ’ includes a large number of viruses and strains. We studied the distribution of the tomato yellow leaf curl disease-associated viruses in Egypt, Israel, Jordan and Lebanon. Two simple and reliable multiplex PCR protocols (mPCR) were developed that allowed the detection of Tomato yellow leaf curl virus (TYLCV) and Tomato yellow leaf curl Sardinia virus (TYLCSV) species, in addition to Tomato yellow leaf curl virus-Mild (TYLCV-Mld) strain. PCR products were sequenced to confirm their identity. The fulllength genomes were also sequenced of TYLCV from