LC DETERMINATION OF ROSUVASTATIN AND EZETIMIBE IN HUMAN PLASMA

A simple, precise, sensitive and validated reverse-phase high performance liquid chromatographic (RP-HPLC) method was developed for the simultaneous estimation of rosuvastatin and ezetimibe in human plasma.The method involved protein precipitation and extraction of both drugs from plasma using acetonitrile and then separation on a C-18 column.Both the analytes were detected at a wavelength of 240 nm using diode array detector.Mobile phase for the said separation consists of a mixture of 1.5% phosphoric acid and acetonitrile in 30:70, v/v ratio.Linearity was in the range of 0.32-267 mg/mL for rosuvastatin and 0.08-67 mg/mL for ezetimibe with coefficient of determination between 0.9997 and 0.9967.Limit of detection was 0.106 mg/mL for rosuvastatin and 0.026 mg/mL for ezetimibe whereas limit of quantification was 0.32 mg/mL and 0.08 mg/mL for rosuvastatin and ezetimibe respectively.Recovery of both the analytes was greater than 75% with RSD less than 15 %.The total run time was less than five min for the two components.The developed method can be fruitfully employed for the determination of these two drugs in human plasma.