Mangiferin in Leaves of Three Thai Mango (Mangifera indica L.) Varieties

Mango is a rich source of biological active compound, mangiferin that is a C-glycosyl xanthone structure. In the present study, mangiferin was isolated from leaves of three mango varieties cultivated in Thailand including: Nam Doc Mai (N), Keow Savoey (K), and Gaew (G). Methanol, ethanol and 70% acetone were used as extraction solvents. Extraction with methanol yielded the highest amount of mangiferin from allof 3 varieties (2.80 %w/w, N; 2.40 %w/w, K; 1.30 %w/w, G), followed by extraction with ethanol and 70% acetone, respectively. And Nam Doc Mai was the variety that provided largest amount of mangiferin. The isolation method for preparing mangiferin in this study could eradicate pigments and other impurities with unsophisticated and inexpensive processes comprising maceration, acid hydrolyzation, partition with organic solvent, and recrystallization.