2013•DergiPark (Istanbul University)Requires access
Comparison of various techniques used for diagnosis of rabies in cats.
Mugale Madhav Nilakanth, B.S. Sandhu, Beigh Akeel, Gupta Kuldeep, Sood Naresh Kumar, Charan Singh, Mugale Mn, Kushagra Gupta, Sood Nk, Akeel Bashir Beigh, Singh Ck
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Abstract
Abstract Mugale MN, Sandhu BS, Gupta K, Sood NK, Beigh A, Singh CK. Comparison of various techniques used for diagnosis of rabies in cats. Eurasian J Vet Sci, 2013, 29, 2, 92-96 Aim: Objectives of this study was to compare and evaluate the best method for diagnosis of rabies in cats. Materials and Methods: Antemortem examination of 5 suspected cats were evaluated. Brains were collected from the suspected cats. Direct Fluorescent Antibody Test (dFAT), Hematoxylin and Eosin (H & E) and Immunohistochemistry (IHC) histopathological diagnostic tests were applied. Results: The dFAT test was conducted on the saliva and fresh brain impression smear of all cats, among that 4 cats showed positive for rabies virus. After death of cats, Hematoxylin & Eosin (H&E) and Immunohistochemistry (IHC) techniques were carried out. One case showed meningitis and remaining showed Negri bodies. Viral antigen depositions were observed by counting 100 cells each region of hippocampus and cerebellum by H&E and IHC. Hippocampus pyramidal cell showed 87% and cerebellum Purkinje cells showed 69% of rabies viral antigen deposition.