Modelling and parameter estimation of the enzymatic synthesis of oligosaccharides by ?-galactosidase fromBacillus circulans

The aim of this research is to develop a model to describe oligosaccharide synthesis and simultaneously lactose hydrolysis. Model A (engineering approach) and model B (biochemical approach) were used to describe the data obtained in batch experiments with beta-galactosidase from Bacillus circulans at various initial lactose concentrations (from 0.19 to 0.59 mol.kg(-1)). A procedure was developed to fit the model parameters and to select the most suitable model. The procedure can also be used for other kinetically controlled reactions. Each experiment was considered as an independent estimation of the model parameters, and consequently, model parameters were fitted to each experiment separately. Estimation of the parameters per experiment preserved the time dependence of the measurements and yielded independent sets of parameters. The next step was to study by ordinary regression methods whether parameters were constant under the altering conditions examined. Throughout all experiments, the parameters of model B did not show a trend upon the initial lactose concentration when inhibition was included. Therefore model B, a galactosyl-enzyme complex-based model, was chosen to describe the oligosaccharide synthesis, and one parameter set was determined for various initial lactose concentrations. Copyright 1999 John Wiley & Sons, Inc.