2006AFRICAN JOURNAL OF BIOTECHNOLOGYOpen access

Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants

F. Pourmorad, Seyed Jalal Hosseinimehr, Naghi Shahabimajd

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Abstract

In present study, we carried out a systematic record of the relative antioxidant activity in selected Iranian medicinal plant species' extracts. The total phenol varied from 24.1 ± 1 to 289.5 ± 5 mg g -1 in the extracts. Flavonoid contents were between 25.15 ± 0.8 and 78.3 ± 4.5 mg g-1. 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging effect of the extracts was determined spectrophotometrically. The highest radical scavenging effect was observed in Mellilotus officinalis with IC50 = 0.018 mg ml –1. The potency of radical scavenging effect of M. officinalis extract was about 4 times greater than synthetic antioxidant butylated hydroxy toluene (BHT). The greater amount of phenolic compounds leads to morepotent radical scavenging effect as shown by M. officinalis extract

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In present study, we carried out a systematic record of the relative antioxidant activity in selected Iranian medicinal plant species' extracts. The total phenol varied from 24.1 ± 1 to 289.5 ± 5 mg g -1 in the extracts. Flavonoid contents were between 25.15 ± 0.8 and 78.3 ± 4.5 mg g-1. 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging effect of the extracts was determined spectrophotometrically. The highest radical scavenging effect was observed in Mellilotus officinalis with IC50 = 0.018 mg ml –1. The potency of radical scavenging effect of M. officinalis extract was about 4 times greater than synthetic antioxidant butylated hydroxy toluene (BHT). The greater amount of phenolic compounds leads to morepotent radical scavenging effect as shown by M. officinalis extract

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Available abstract

In present study, we carried out a systematic record of the relative antioxidant activity in selected Iranian medicinal plant species' extracts. The total phenol varied from 24.1 ± 1 to 289.5 ± 5 mg g -1 in the extracts. Flavonoid contents were between 25.15 ± 0.8 and 78.3 ± 4.5 mg g-1. 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging effect of the extracts was determined spectrophotometrically. The highest radical scavenging effect was observed in Mellilotus officinalis with IC50 = 0.018 mg ml –1. The potency of radical scavenging effect of M. officinalis extract was about 4 times greater than synthetic antioxidant butylated hydroxy toluene (BHT). The greater amount of phenolic compounds leads to morepotent radical scavenging effect as shown by M. officinalis extract

Key concepts: Chemistry, DPPH, Antioxidant, Phenol, Flavonoid, Scavenging, Officinalis, Phenols

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