2005•Protein and Peptide LettersRequires access

Efficient Immobilization of Enzymes on Microchannel Surface Through His-Tag and Application for Microreactor

Masaya Miyazaki, Jun Kaneno, Susumu Yamaori, Takeshi Honda, Maria Portia P. Briones, Masato Uehara, Kazunari Arima, Ken-ichi Kan’no, Kenichi Yamashita, Yoshiko Yamaguchi, Hiroyuki Nakamura, Hiroo Yonezawa, Masayuki Fujii, Hideaki Maeda

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Abstract

We developed a simple immobilisation method for His-tagged enzymes on a microchannel surface. It facilitates immobilisation of protein molecule on microchannel surface through Ni-complex, using crude or purified protein solutions. By this method, we could immobilize proteins on microcapillary constantly. This method might be useful for further development of microreactor with reversibly immobilized enzymes. Keywords: reversible immobilization, surface modification, microreactor, enzyme reaction

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Key concepts: Microreactor, Microchannel, Enzyme, Chemistry, Microfluidics, Surface modification, Immobilized enzyme, Nanotechnology