Simultaneous Determination of Three Kinds of Iridoids Compounds in Eucommia ulmoides by RP-HPLC

A simple and rapid analytical method of the three iridoids compounds, geniposidic acid (GPA), geniposide (GP) and aucubin (AU), which are naturally occurring bioactives were determined in Eucommia samples simultaneously by isocratic reverse phase high performance liquid chromatograph (RP-HPLC) using UV detection. All of these iridoids were identified with standard samples. A C18 column was used as the stationary phase, and the mobile phase was methanol-water-acetic acid=24.5:75:0.5 (V/V/V) at the flow rate of 1ml · min-1. The detection wavelength was set at 220 nm, and the column temperature was 25 °C. Three calibration curves were obtained, and the mean recoveries of GPA, GP and AU were 99.10%, 98.06% and 96.07% respectively.