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1993•FEMS Microbiology LettersRequires access

Fast classification of plant-associated bacteria in theXanthomonasgenus

Martine Maes

Open publisher page 57 citations

Abstract

A rapid method for identifying Xanthomonas bacteria was developed, based on specific PCR amplification. In all Xanthomonas pathovars tested, a single 16S rDNA fragment of 480 bp was produced. Phytobacteria from other genera showed other fragment patterns on agarose gel electrophoresis. This Xanthomonas identification system was used to screen Xanthomonas-related organisms, newly isolated phytobacteria and bacterial populations in wheat seed extracts.

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What this paper is about

A rapid method for identifying Xanthomonas bacteria was developed, based on specific PCR amplification. In all Xanthomonas pathovars tested, a single 16S rDNA fragment of 480 bp was produced. Phytobacteria from other genera showed other fragment patterns on agarose gel electrophoresis. This Xanthomonas identification system was used to screen Xanthomonas-related organisms, newly isolated phytobacteria and bacterial populations in wheat seed extracts.

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OpenAlex reports 57 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

A rapid method for identifying Xanthomonas bacteria was developed, based on specific PCR amplification. In all Xanthomonas pathovars tested, a single 16S rDNA fragment of 480 bp was produced. Phytobacteria from other genera showed other fragment patterns on agarose gel electrophoresis. This Xanthomonas identification system was used to screen Xanthomonas-related organisms, newly isolated phytobacteria and bacterial populations in wheat seed extracts.

Key concepts: Xanthomonas, Biology, Bacteria, Agarose gel electrophoresis, 16S ribosomal RNA, Microbiology, Polymerase chain reaction, Pseudomonadaceae

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