RAPD (random amplified polymorphic DNA) marker as a tool for hybrid oil palm verification from half mature zygotic embryo culture

Hybrid verifications within the F I population (Tenera) between Pisifera (72, 110, 172, 206, 558 and 777) and Dura (366 and 865) were detected by randomly amplified polymorphic DNA (RAPD) analysis. Firstly, DNA from half mature zygotic embryo of six combinations of hybrid were isolated and detected by RAPD using 7 arbitrary 10-mer primers. From all random primers tested they could amplify parental DNA. Secondly, half mature zygotic embryo consisted of coleoptiles region of those combinations was cultured on Murashige and Skoog (MS) medium plus various kinds and concentrations of auxins. The result revealed that OPT06 primers provided clearly DNA pattern and could be used for hybrid verification of the crosses 865 (D) x 206 (P). The highest frequency of callus formation (46.60%) was obtained on the medium supplemented with 2.50 mg/l 3,6-dichloro-o-anisic acid (dicamba) containing MS medium, significant difference to other kinds and concentrations of auxins.