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1975•Journal of NeurochemistryRequires access

AXONAL TRANSPORT OF LABELLED PROTEINS IN SENSORY FIBRES OF RABBIT VAGUS NERVE IN VITRO

W. Graham McLean, M. Frizell, J. Sjöstrand

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Abstract

Abstract —Rabbit vagus nerves and nodose ganglia were incubated in vitro for up to 24 h in two‐compartment chambers. After the introduction of [3H]leucine or [3H]fucose to the ganglion compartments a rapid anterograde axonal transport of labelled proteins or glycoproteins occurred at rates of 330 ± 44 mm/day and 336 ± 30 mm/day respectively. Accumulation of [3H]leucine‐labelled proteins proximal to a ligature on the nerve was unaffected by a delay of up to 6 h between removal of the nerve and labelling in vitro. Accumulation was prevented by inhibition of protein synthesis in the ganglion but not in the axon and was inhibited in a graded manner by colchicine.

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Abstract —Rabbit vagus nerves and nodose ganglia were incubated in vitro for up to 24 h in two‐compartment chambers. After the introduction of [3H]leucine or [3H]fucose to the ganglion compartments a rapid anterograde axonal transport of labelled proteins or glycoproteins occurred at rates of 330 ± 44 mm/day and 336 ± 30 mm/day respectively. Accumulation of [3H]leucine‐labelled proteins proximal to a ligature on the nerve was unaffected by a delay of up to 6 h between removal of the nerve and labelling in vitro. Accumulation was prevented by inhibition of protein synthesis in the ganglion but not in the axon and was inhibited in a graded manner by colchicine.

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Available abstract

Abstract —Rabbit vagus nerves and nodose ganglia were incubated in vitro for up to 24 h in two‐compartment chambers. After the introduction of [3H]leucine or [3H]fucose to the ganglion compartments a rapid anterograde axonal transport of labelled proteins or glycoproteins occurred at rates of 330 ± 44 mm/day and 336 ± 30 mm/day respectively. Accumulation of [3H]leucine‐labelled proteins proximal to a ligature on the nerve was unaffected by a delay of up to 6 h between removal of the nerve and labelling in vitro. Accumulation was prevented by inhibition of protein synthesis in the ganglion but not in the axon and was inhibited in a graded manner by colchicine.

Key concepts: Nodose Ganglion, Axoplasmic transport, Vagus nerve, Axon, In vitro, Colchicine, Ganglion, Leucine

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AXONAL TRANSPORT OF LABELLED PROTEINS IN SENSORY FIBRES OF RABBIT VAGUS NERVE IN VITRO — Research Paper | ScholarLens