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1988•Journal of Experimental BotanyRequires access

The Measurement of Ribulose 1, 5-bisphosphate Carboxylase/Oxygenase Concentration in the Leaves of Potato Plants by Enzyme Linked Immunosorbtion Assays

James Catt, Peter Millard

Open publisher page 27 citations

Abstract

Catt, J. W. and Millard, P. 1988. The measurement of ribulose 1, 5-bisyphosphate carboxylase/ oxygenase concentration in the leaves of potato plants by enzyme linked immunosorbtion assays.—J. exp. Bot. 39: 157-164. An enzyme linked immunosorbtion assay (ELISA) has been developed for the measurement of ribulose l, 5-bistfphosphate carboxylase/oxygenase (RUBISCO) concentrations in potato leaves. Extracts of leaves were made using a buffer containing detergent and used to coat ELISA plates. The amount of RUBISCO binding was estimated using a double antibody technique. Various methods of extraction were used and assessed and the efficiency of the assay was tested using purified RUBISCO. The method is applicable to the determination of RUBISCO from diverse sources.

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Catt, J. W. and Millard, P. 1988. The measurement of ribulose 1, 5-bisyphosphate carboxylase/ oxygenase concentration in the leaves of potato plants by enzyme linked immunosorbtion assays.—J. exp. Bot. 39: 157-164. An enzyme linked immunosorbtion assay (ELISA) has been developed for the measurement of ribulose l, 5-bistfphosphate carboxylase/oxygenase (RUBISCO) concentrations in potato leaves. Extracts of leaves were made using a buffer containing detergent and used to coat ELISA plates. The amount of RUBISCO binding was estimated using a double antibody technique. Various methods of extraction were used and assessed and the efficiency of the assay was tested using purified RUBISCO. The method is applicable to the determination of RUBISCO from diverse sources.

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Available abstract

Catt, J. W. and Millard, P. 1988. The measurement of ribulose 1, 5-bisyphosphate carboxylase/ oxygenase concentration in the leaves of potato plants by enzyme linked immunosorbtion assays.—J. exp. Bot. 39: 157-164. An enzyme linked immunosorbtion assay (ELISA) has been developed for the measurement of ribulose l, 5-bistfphosphate carboxylase/oxygenase (RUBISCO) concentrations in potato leaves. Extracts of leaves were made using a buffer containing detergent and used to coat ELISA plates. The amount of RUBISCO binding was estimated using a double antibody technique. Various methods of extraction were used and assessed and the efficiency of the assay was tested using purified RUBISCO. The method is applicable to the determination of RUBISCO from diverse sources.

Key concepts: Pyruvate carboxylase, Oxygenase, Ribulose, Enzyme, RuBisCO, Botany, Chemistry, Biology

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The Measurement of Ribulose 1, 5-bisphosphate Carboxylase/Oxygenase Concentration in the Leaves of Potato Plants by Enzyme Linked Immunosorbtion Assays — Research Paper | ScholarLens