흰쥐 대뇌세포의 저산소증 모델에서 석창포(石菖蒲 Acori graminei rhizoma. AGR)에 의한 유전자 표현 변화의 microarray 분석

Acori graminei Rhizoma (AGR) is a perennial herb which has been used clinically as a traditional oriental medicine against stroke, Alzheimer’s disease, and vascular dementia. We investigated the effect of AGR on the modulation of gene expression profile in a hypoxic model of cultured rat cortical cells. Rat cerebrocortical cells were grown in Neurobasal medium. On DIV12, cells were treated with AGR (10 ㎍/㎖), given a hypoxic shock (2% O₂, 3 hr) on DIV14, and total RNAs were prepared one day after shock. Microarray analyses indicated that the expression levels of most genes were altered within the global M values +0.5 and -0.5, i.e., 40% increase or decrease. There were 750 genes which were upregulated by ＜ global M +0.2, while 700 genes were downregulated by ＞ global M -0.2. The overall profile of gene expression suggests that AGR suppresses apoptosis (upregulation of anti-apopotic genes such as TEGT, TIEG, Dad, p53, and downregulation of pro-apopotic genes such as DAPK, caspase 2, pdcd8), ROS (upregulation of RARα, AhR), and that AGR has neurotrophic effects (upregulation of Akt1, Akt2). These results provide a platform for investigation of the molecular mechanism of the effect of AGR in neuroprotection.