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1994•Journal of Analytical ToxicologyRequires access

Determination of Basic Drugs Extracted from Biological Matrices by Means of Solid-Phase Extraction and Wide-Bore Capillary Gas Chromatography with Nitrogen-Phosphorus Detection

Xiaohua Chen, Jan‐Piet Franke, Jaap Wijsbeek, Rokus A. de Zeeuw

Open publisher page 34 citations

Abstract

Determination of basic drugs from biological matrices at concentration levels of 100-200 ng/mL was accomplished by using mixed-mode Bond Elut Certify columns and a gas chromatograph equipped with a nitrogen-phosphorus detector. The extraction procedure developed for general drug screening on a GC-FID system was found suitable for the extraction of basic drugs from plasma and whole blood for GC-NPD analysis. For urine samples, an extra wash step with 20% acetonitrile in water was required to obtain clean extracts. The recoveries of 18 basic drugs ranged from 77.7 to 104.5%, with relative standard deviations less than 7.0%.

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Determination of basic drugs from biological matrices at concentration levels of 100-200 ng/mL was accomplished by using mixed-mode Bond Elut Certify columns and a gas chromatograph equipped with a nitrogen-phosphorus detector. The extraction procedure developed for general drug screening on a GC-FID system was found suitable for the extraction of basic drugs from plasma and whole blood for GC-NPD analysis. For urine samples, an extra wash step with 20% acetonitrile in water was required to obtain clean extracts. The recoveries of 18 basic drugs ranged from 77.7 to 104.5%, with relative standard deviations less than 7.0%.

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Available abstract

Determination of basic drugs from biological matrices at concentration levels of 100-200 ng/mL was accomplished by using mixed-mode Bond Elut Certify columns and a gas chromatograph equipped with a nitrogen-phosphorus detector. The extraction procedure developed for general drug screening on a GC-FID system was found suitable for the extraction of basic drugs from plasma and whole blood for GC-NPD analysis. For urine samples, an extra wash step with 20% acetonitrile in water was required to obtain clean extracts. The recoveries of 18 basic drugs ranged from 77.7 to 104.5%, with relative standard deviations less than 7.0%.

Key concepts: Chromatography, Chemistry, Extraction (chemistry), Gas chromatography, Solid phase extraction, Urine, Capillary action, Phosphorus

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