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Practical Applications of the FISH Technique

George Kontogeorgos, Nikiforos Kapranos, Eleni Thodou

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Abstract

Fluorescence in situ hybridization (FISH), often referred to as molecular cytogenetics, is currently recognized as a reliable technique and an alternative to classic cytogenetics (karyotyping), with many applications for detecting chromosomal abnormalities. The technique can analyze interphase nuclei of resting cells or metaphase spreads of mitotic cells. In addition, specific protocols for karyotyping analysis combine FISH with classic cytogenetics.

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What this paper is about

Fluorescence in situ hybridization (FISH), often referred to as molecular cytogenetics, is currently recognized as a reliable technique and an alternative to classic cytogenetics (karyotyping), with many applications for detecting chromosomal abnormalities. The technique can analyze interphase nuclei of resting cells or metaphase spreads of mitotic cells. In addition, specific protocols for karyotyping analysis combine FISH with classic cytogenetics.

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Available abstract

Fluorescence in situ hybridization (FISH), often referred to as molecular cytogenetics, is currently recognized as a reliable technique and an alternative to classic cytogenetics (karyotyping), with many applications for detecting chromosomal abnormalities. The technique can analyze interphase nuclei of resting cells or metaphase spreads of mitotic cells. In addition, specific protocols for karyotyping analysis combine FISH with classic cytogenetics.

Key concepts: Cytogenetics, Metaphase, Fish <Actinopterygii>, Molecular cytogenetics, Karyotype, Fluorescence in situ hybridization, Biology, Interphase

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