Biomolecular assembly strategies to develop potential artificial cellulosomes

Abstract Cellulosic biomass is a sustainable source for fuels and value-added chemicals, and is available in large quantities. One of the key challenges in biomass processing is associated with the establishment of an efficient enzymatic degradation of plant cell wall. A multi-enzymatic complex, cellulosome, was identified as a highly efficient biocatalyst for the hydrolysis of cellulosic biomass in nature. Significant progress has been achieved on cellulosome production and application since its discovery, but there is still a gap for industrial use. Artificial systems are being developed by employing various pairs of proteins and scaffolds with the objective of reconstructing this natural multi-enzymatic complex for sustainable biotechnology application.