2014•American-Asian-Journal of agricultural & environmental sciences/American-Eurasian journal of agricultural & environmental sciencesRequires access
Optimal conditions for high-yield protoplast isolations of Jatropha curcas L. and Ricinus communis L.
Nootjaree Tudses, Siripong Premjet, Duangporn Premjet
Open publisher page 6 citations
Abstract
The purpose of this study was to investigate the optimal conditions for high-yield protoplast isolation of Jatropha curcas L. and Ricinus communis L. for producing an intergeneric somatic hybrid. To isolate a large amount of protoplasts from J. curcas L. and R. communis L., several factors that affect protoplast isolation, such as the source of the plant tissue, type of cellulase, enzyme combination, incubation time and osmoticum were investigated. Our results indicated that in vitro leaves yielded a higher number of protoplasts than calli. Cellulase onozuka R10 highly affected protoplast isolation. In addition, 14 enzyme combinations of cellulase (Aspergillus sp.), cellulase onozuka R10, pectinase and pectolyase Y23 yielded various amounts of protoplasts. The greatest yield (94.5 ± 4.5 × 10 protoplasts/gFW) and viability (77.8 ± 3.1%) of J. curcas L. protoplasts was obtained from cellulase 5