2000•ScienceRequires access
A BAC-Based Physical Map of the Major Autosomes of Drosophila melanogaster
Roger A. Hoskins, Catherine R. Nelson, Benjamin Paul Berman, Todd Laverty, Reed A. George, L. D. Ciesiolka, Mohammed Naeemuddin, Andrew D. Arenson, James Durbin, Robert David, Paul E. Tabor, Michael R. P. Bailey, Denise R. Deshazo, Joseph J. Catanese, Aaron Gerald Mammoser, Kazutoyo Osoegawa, † Pieter J. de, Jong, S Celniker, Richard A. Gibbs, Gerald M. Rubin, Steven E. Scherer
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Abstract
We constructed a bacterial artificial chromosome (BAC)-based physical map of chromosomes 2 and 3 of Drosophila melanogaster, which constitute 81% of the genome. Sequence tagged site (STS) content, restriction fingerprinting, and polytene chromosome in situ hybridization approaches were integrated to produce a map spanning the euchromatin. Three of five remaining gaps are in repeat-rich regions near the centromeres. A tiling path of clones spanning this map and STS maps of chromosomes X and 4 was sequenced to low coverage; the maps and tiling path sequence were used to support and verify the whole-genome sequence assembly, and tiling path BACs were used as templates in sequence finishing.