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2003•Journal of Clinical MicrobiologyRequires access

Amplified-Fragment Length Polymorphism as a Complement to IS 6110 -Based Restriction Fragment Length Polymorphism Analysis for Molecular Typing of Mycobacterium tuberculosis

Monserrat Ruiz, Juan Carlos Rodrı́guez, Francisco Rodríguez‐Valera, Gloria Royo

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Abstract

The amplified-fragment length polymorphism (AFLP) technique was applied to clusters of Mycobacterium tuberculosis clinical isolates obtained by using IS6110-based restriction fragment length polymorphism (RFLP). Ten of the RFLP clusters showed identical AFLP patterns also, but the other 13 could be resolved into subclusters by AFLP. Our results suggest that some RFLP clusters may not be due to recent transmission and that AFLP may be a useful complementary technique.

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The amplified-fragment length polymorphism (AFLP) technique was applied to clusters of Mycobacterium tuberculosis clinical isolates obtained by using IS6110-based restriction fragment length polymorphism (RFLP). Ten of the RFLP clusters showed identical AFLP patterns also, but the other 13 could be resolved into subclusters by AFLP. Our results suggest that some RFLP clusters may not be due to recent transmission and that AFLP may be a useful complementary technique.

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Available abstract

The amplified-fragment length polymorphism (AFLP) technique was applied to clusters of Mycobacterium tuberculosis clinical isolates obtained by using IS6110-based restriction fragment length polymorphism (RFLP). Ten of the RFLP clusters showed identical AFLP patterns also, but the other 13 could be resolved into subclusters by AFLP. Our results suggest that some RFLP clusters may not be due to recent transmission and that AFLP may be a useful complementary technique.

Key concepts: Amplified fragment length polymorphism, Restriction fragment length polymorphism, Genetics, Biology, Terminal restriction fragment length polymorphism, Polymorphism (computer science), Cleaved amplified polymorphic sequence, Typing

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Amplified-Fragment Length Polymorphism as a Complement to IS 6110 -Based Restriction Fragment Length Polymorphism Analysis for Molecular Typing of Mycobacterium tuberculosis — Research Paper | ScholarLens